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A formulation of liquid media for mushroom spawn production, which comprises glucose, yeast extract, dipotassium hydrogen orthophosphate, potassium dihydrogen phosphate, magnesium sulphate, peptone and water. The disclosure also provides a method to produce liquid spawn of mushroom, comprising steps of preparing the said liquid media, sterilizing the liquid media, inoculating a fungal mycelium or spore into the sterile liquid media, and culturing the fungus in the sterile liquid media.
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